HGC-27-CAS9 cell line

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HGC-27-CAS9 Order

HGC-27-CAS9 cell line
Catalog#
YC-C079-Cas9-H
Size
1*10^6
Transfection Culture Medium
Transfection Culture medium-B
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
HGC-27-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
HGC-27-CAS9
Cell Morphology
Endothelial cells, adherent
Passage ratio
1:3~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10% FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the HGC-27 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HGC-27-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
HGC-27-CAS9 Cas9 19.70162010 5.17640495
HGC-27-CAS9 β-actin 14.52521515
HGC-27 Cas9 33.80643082 19.62654114
HGC-27 β-actin 14.17988968
Cutting Efficiency Validation
HGC-27-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the HGC-27-CAS9 stable cell pool which is electroporated by PIN1 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HGC-27		 STR Info (Cell bank)
Cell Line:HGC-27
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 10111011
D7S820 111213.0111213.0
D16S539 10111011
VWA 14141414
TH01 9999
AMEL XXXX
TPOX 8888
CSF1PO 12121212
D12S391 2021
FGA 2222
D2S1338 2224
D21S11 303334.0
D18S51 1617
D8S1179 1111
D3S1358 1717
D6S1043 1818
PENTAE 1818
D19S433 1414
PENTAD 913
D1S1656 1416
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
HGC-27-CAS9 cell line
HGC-27-CAS9
Catalog#
YC-C079-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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